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Each vial contained 10 flies with a fixed 1:1 sex ratio (5 females, 5 males) across all treatment conditions, so density and sex composition were held constant

The activity of glutathione S-transferase (GST, EC 2.5.1.18) was determined in the extracts obtained after yeast disintegration process by using monochlorobimane (MCB) as the substrate (BioVision, Mountain View, CA, USA), which reacts with glutathione to form a fluorescent colored adduct

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Reference intake of an averageadult (8400 kJ /2000 kcal)

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If you are low in Vitamin B12 or have a deficiency anaemia, you will not be creating enough red blood cells and the ones you do make might be defective, being abnormally large