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For example, it has been observed that the expression levels of four TRP channels (TRPA1, TRPM2, TRPV1 and TRPV4) are high in neurons related to nociception

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Subsequently, the BD Rhapsody analysis process was used for quality control and filtering of data, identification of cell ID and molecule ID, mapping of reads to the reference genome, quantification of gene expression and identification of cells, and, finally, generation of the original expression matrix

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The protein was purified in a similar procedure as described above